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1
treatment: siNT,cell line: OCI-LY1
413 E 69th Street, BB-1462
New York
USA
WCMC
Katerina,,Hatzi
ChIP-seq: Raw images generated went through primary image analysis and basecalling (RTA v1.6) that was followed by Illumina Genome Analyzer Off-Line Basecaller (OLB v1.6) analysis, where reads were aligned to the human genome (UCSC hg18) using ELAND. Only sequences mapped uniquely to the genome with not more than 2 mis...
ChIP-seq libraries were prepared using the Illumina ChIP-seq Library preparation Kit following the manufacture’s instructions with minor modifications. Briefly 10ng of purified ChIP DNA was end repaired by conversion of overhangs to phosphorylated blunt ends. A’ bases were added to the 3’ends of the DNA fragments...
GSM1000981
Illumina HiSeq 1000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL15433
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX185895,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01163911,Named Annotation: NA000044218.1 (GSM1000981_OCI-LY1_48hrs_mRNAseq_3x_siNT_R1.bw)
GSM1000981
GSE29282
0
Human DLBCL cel line
Public on Aug 05 2013
Sep 10 2012
9606
OCI-LY1_48hrs_mRNAseq_3x_siNT_R1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX185895
https://www.ncbi.nlm.nih.gov/biosample/SAMN01163911
1
treatment: siNT,cell line: OCI-LY1
413 E 69th Street, BB-1462
New York
USA
WCMC
Katerina,,Hatzi
ChIP-seq: Raw images generated went through primary image analysis and basecalling (RTA v1.6) that was followed by Illumina Genome Analyzer Off-Line Basecaller (OLB v1.6) analysis, where reads were aligned to the human genome (UCSC hg18) using ELAND. Only sequences mapped uniquely to the genome with not more than 2 mis...
ChIP-seq libraries were prepared using the Illumina ChIP-seq Library preparation Kit following the manufacture’s instructions with minor modifications. Briefly 10ng of purified ChIP DNA was end repaired by conversion of overhangs to phosphorylated blunt ends. A’ bases were added to the 3’ends of the DNA fragments...
GSM1000982
Illumina HiSeq 1000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL15433
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX185896,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01163912,Named Annotation: NA000044219.1 (GSM1000982_OCI-LY1_48hrs_mRNAseq_3x_siNT_R2.bw)
GSM1000982
GSE29282
0
Human DLBCL cel line
Public on Aug 05 2013
Sep 10 2012
9606
OCI-LY1_48hrs_mRNAseq_3x_siNT_R2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX185896
https://www.ncbi.nlm.nih.gov/biosample/SAMN01163912
1
treatment: siNT,cell line: OCI-LY1
413 E 69th Street, BB-1462
New York
USA
WCMC
Katerina,,Hatzi
ChIP-seq: Raw images generated went through primary image analysis and basecalling (RTA v1.6) that was followed by Illumina Genome Analyzer Off-Line Basecaller (OLB v1.6) analysis, where reads were aligned to the human genome (UCSC hg18) using ELAND. Only sequences mapped uniquely to the genome with not more than 2 mis...
ChIP-seq libraries were prepared using the Illumina ChIP-seq Library preparation Kit following the manufacture’s instructions with minor modifications. Briefly 10ng of purified ChIP DNA was end repaired by conversion of overhangs to phosphorylated blunt ends. A’ bases were added to the 3’ends of the DNA fragments...
GSM1000983
Illumina HiSeq 1000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL15433
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX185897,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01163913,Named Annotation: NA000044220.1 (GSM1000983_OCI-LY1_48hrs_mRNAseq_3x_siNT_R3.bw)
GSM1000983
GSE29282
0
Human DLBCL cel line
Public on Aug 05 2013
Sep 10 2012
9606
OCI-LY1_48hrs_mRNAseq_3x_siNT_R3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX185897
https://www.ncbi.nlm.nih.gov/biosample/SAMN01163913
1
treatment: siBCL6,cell line: OCI-LY1
413 E 69th Street, BB-1462
New York
USA
WCMC
Katerina,,Hatzi
ChIP-seq: Raw images generated went through primary image analysis and basecalling (RTA v1.6) that was followed by Illumina Genome Analyzer Off-Line Basecaller (OLB v1.6) analysis, where reads were aligned to the human genome (UCSC hg18) using ELAND. Only sequences mapped uniquely to the genome with not more than 2 mis...
ChIP-seq libraries were prepared using the Illumina ChIP-seq Library preparation Kit following the manufacture’s instructions with minor modifications. Briefly 10ng of purified ChIP DNA was end repaired by conversion of overhangs to phosphorylated blunt ends. A’ bases were added to the 3’ends of the DNA fragments...
GSM1000984
Illumina HiSeq 1000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL15433
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX185898,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01163914,Named Annotation: NA000044221.1 (GSM1000984_OCI-LY1_48hrs_mRNAseq_3x_siBCL6_R1.bw)
GSM1000984
GSE29282
0.004366
Human DLBCL cel line
Public on Aug 05 2013
Sep 10 2012
9606
OCI-LY1_48hrs_mRNAseq_3x_siBCL6_R1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX185898
https://www.ncbi.nlm.nih.gov/biosample/SAMN01163914
1
treatment: siBCL6,cell line: OCI-LY1
413 E 69th Street, BB-1462
New York
USA
WCMC
Katerina,,Hatzi
ChIP-seq: Raw images generated went through primary image analysis and basecalling (RTA v1.6) that was followed by Illumina Genome Analyzer Off-Line Basecaller (OLB v1.6) analysis, where reads were aligned to the human genome (UCSC hg18) using ELAND. Only sequences mapped uniquely to the genome with not more than 2 mis...
ChIP-seq libraries were prepared using the Illumina ChIP-seq Library preparation Kit following the manufacture’s instructions with minor modifications. Briefly 10ng of purified ChIP DNA was end repaired by conversion of overhangs to phosphorylated blunt ends. A’ bases were added to the 3’ends of the DNA fragments...
GSM1000985
Illumina HiSeq 1000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL15433
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX185899,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01163915,Named Annotation: NA000044222.1 (GSM1000985_OCI-LY1_48hrs_mRNAseq_3x_siBCL6_R2.bw)
GSM1000985
GSE29282
0
Human DLBCL cel line
Public on Aug 05 2013
Sep 10 2012
9606
OCI-LY1_48hrs_mRNAseq_3x_siBCL6_R2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX185899
https://www.ncbi.nlm.nih.gov/biosample/SAMN01163915
1
treatment: siBCL6,cell line: OCI-LY1
413 E 69th Street, BB-1462
New York
USA
WCMC
Katerina,,Hatzi
ChIP-seq: Raw images generated went through primary image analysis and basecalling (RTA v1.6) that was followed by Illumina Genome Analyzer Off-Line Basecaller (OLB v1.6) analysis, where reads were aligned to the human genome (UCSC hg18) using ELAND. Only sequences mapped uniquely to the genome with not more than 2 mis...
ChIP-seq libraries were prepared using the Illumina ChIP-seq Library preparation Kit following the manufacture’s instructions with minor modifications. Briefly 10ng of purified ChIP DNA was end repaired by conversion of overhangs to phosphorylated blunt ends. A’ bases were added to the 3’ends of the DNA fragments...
GSM1000986
Illumina HiSeq 1000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL15433
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX185900,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01163916,Named Annotation: NA000044223.1 (GSM1000986_OCI-LY1_48hrs_mRNAseq_3x_siBCL6_R3.bw)
GSM1000986
GSE29282
0
Human DLBCL cel line
Public on Aug 05 2013
Sep 10 2012
9606
OCI-LY1_48hrs_mRNAseq_3x_siBCL6_R3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX185900
https://www.ncbi.nlm.nih.gov/biosample/SAMN01163916
1
spike-in hsa-mir-147: 0.1,spike-in hsa-mir-211: 1,spike-in hsa-mir-219-5p: 10,spike-in hsa-mir-338-3p: 0.1,spike-in hsa-mir-383: 1,spike-in hsa-mir-429: 10,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-m...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002540
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186158,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166092
GSM1002540
GSE40819,GSE40820
0.00513
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix1-rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186158
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166092
1
spike-in hsa-mir-147: 1,spike-in hsa-mir-211: 10,spike-in hsa-mir-219-5p: 0.1,spike-in hsa-mir-338-3p: 1,spike-in hsa-mir-383: 10,spike-in hsa-mir-429: 0.1,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-m...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002541
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186159,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166093
GSM1002541
GSE40819,GSE40820
0
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix2-rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186159
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166093
1
spike-in hsa-mir-147: 10,spike-in hsa-mir-211: 0.1,spike-in hsa-mir-219-5p: 1,spike-in hsa-mir-338-3p: 10,spike-in hsa-mir-383: 0.1,spike-in hsa-mir-429: 1,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-m...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002542
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186160,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166094
GSM1002542
GSE40819,GSE40820
0.130026
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix3-rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186160
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166094
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 0.1,o-methyl spike-in hsa-mir-211: 1,o-methyl spike-in hsa-mir-219-5p: 10,precursor spike-in hsa-mir-338-3p: 0.1,precursor spike-in hsa-mi...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002543
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186161,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166095
GSM1002543
GSE40819,GSE40820
0.143913
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix4-rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186161
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166095
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 1,o-methyl spike-in hsa-mir-211: 10,o-methyl spike-in hsa-mir-219-5p: 0.1,precursor spike-in hsa-mir-338-3p: 1,precursor spike-in hsa-mir-...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002544
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186162,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166096
GSM1002544
GSE40819,GSE40820
0.095942
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix5-rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186162
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166096
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 10,o-methyl spike-in hsa-mir-211: 0.1,o-methyl spike-in hsa-mir-219-5p: 1,precursor spike-in hsa-mir-338-3p: 10,precursor spike-in hsa-mir...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002545
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186163,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166097
GSM1002545
GSE40819,GSE40820
0.161421
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix6-rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186163
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166097
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-mir-383...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002546
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186164,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166098
GSM1002546
GSE40819,GSE40820
0.092059
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix7-rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186164
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166098
1
spike-in hsa-mir-147: 0.1,spike-in hsa-mir-211: 1,spike-in hsa-mir-219-5p: 10,spike-in hsa-mir-338-3p: 0.1,spike-in hsa-mir-383: 1,spike-in hsa-mir-429: 10,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-m...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002547
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186165,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166099
GSM1002547
GSE40819,GSE40820
0
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix1-rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186165
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166099
1
spike-in hsa-mir-147: 1,spike-in hsa-mir-211: 10,spike-in hsa-mir-219-5p: 0.1,spike-in hsa-mir-338-3p: 1,spike-in hsa-mir-383: 10,spike-in hsa-mir-429: 0.1,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-m...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002548
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186166,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166100
GSM1002548
GSE40819,GSE40820
0.049992
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix2-rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186166
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166100
1
spike-in hsa-mir-147: 10,spike-in hsa-mir-211: 0.1,spike-in hsa-mir-219-5p: 1,spike-in hsa-mir-338-3p: 10,spike-in hsa-mir-383: 0.1,spike-in hsa-mir-429: 1,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-m...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002549
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186167,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166101
GSM1002549
GSE40819,GSE40820
0.107731
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix3-rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186167
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166101
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 0.1,o-methyl spike-in hsa-mir-211: 1,o-methyl spike-in hsa-mir-219-5p: 10,precursor spike-in hsa-mir-338-3p: 0.1,precursor spike-in hsa-mi...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002550
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186168,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166102
GSM1002550
GSE40819,GSE40820
0
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix4-rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186168
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166102
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 1,o-methyl spike-in hsa-mir-211: 10,o-methyl spike-in hsa-mir-219-5p: 0.1,precursor spike-in hsa-mir-338-3p: 1,precursor spike-in hsa-mir-...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002551
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186169,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166103
GSM1002551
GSE40819,GSE40820
0.02033
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix5-rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186169
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166103
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 10,o-methyl spike-in hsa-mir-211: 0.1,o-methyl spike-in hsa-mir-219-5p: 1,precursor spike-in hsa-mir-338-3p: 10,precursor spike-in hsa-mir...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002552
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186170,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166104
GSM1002552
GSE40819,GSE40820
0.143934
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix6-rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186170
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166104
1
spike-in hsa-mir-147: 0,spike-in hsa-mir-211: 0,spike-in hsa-mir-219-5p: 0,spike-in hsa-mir-338-3p: 0,spike-in hsa-mir-383: 0,spike-in hsa-mir-429: 0,precursor spike-in hsa-mir-147: 0,o-methyl spike-in hsa-mir-211: 0,o-methyl spike-in hsa-mir-219-5p: 0,precursor spike-in hsa-mir-338-3p: 0,precursor spike-in hsa-mir-383...
P.O.B. 26
Rehovot
Israel
Weizmann Institute of Science
Dena,,Leshkowitz
Basecalls performed using CASAVA version 1.8.1,Adaptors were removed and then collapsed into tags and quantified.,Tags that appeared at a frequency of 5 or more reads per library were normalized to reads per million (rpm).,The tags were annotated using the best hit of BLAST 2.2.23 (-S -e 0.01 options) and run against h...
Commercial human placenta RNA was purchased from Ambion (AM7950) in two lots: 03070032 and 1004005. Small RNA library construction from 5 ug total RNA of each of the 14 samples was carried out using illumina TruSeq smRNA Sample Prep Kit (San-Diego, CA, USA), according to the manufacturer’s instructions. Briefly, 3` a...
GSM1002553
Illumina HiSeq 2000
May 15 2019
size fractionation
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186171,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01166105
GSM1002553
GSE40819,GSE40820
0.03988
placenta
Public on Mar 01 2013
Sep 12 2012
9606
mix7-rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186171
https://www.ncbi.nlm.nih.gov/biosample/SAMN01166105
1
strategy: iCLIP-Seq,cell line: HeLa,clip antibody: CstF64
B228 Med Sci I
Irvine
USA
University of California, Irvine
Yongsheng,,Shi
iCLIP-seq: 1. Raw reads were demultiplexed using the sequencing barcode unique to each replicate and an additional random trinucleotide identifying individual DNA molecules was clipped but kept as metadata; 2. Reads with quality less than 20 for 10% or more of the bases were removed.3. We mapped the remaining reads to ...
iCLIP-seq: RNAs were extracted from immunoprecipitated protein-RNA complexes; for direct RNA sequencing, RNAs were extracted from cells using Trizol reagent (Invitrogen),iCLIP-seq libraries were prepared as described by Konig et al, NSMB (2010) 17: 909; the antibody used was purchased from Bethyl Laboratories (Cat#: A3...
GSM1003587
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186536,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01173953
GSM1003587
GSE40859
0.064095
HeLa
Public on Oct 01 2012
Sep 13 2012
9606
CstF64-iCLIP rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186536
https://www.ncbi.nlm.nih.gov/biosample/SAMN01173953
1
strategy: iCLIP-Seq,cell line: HeLa,clip antibody: CstF64
B228 Med Sci I
Irvine
USA
University of California, Irvine
Yongsheng,,Shi
iCLIP-seq: 1. Raw reads were demultiplexed using the sequencing barcode unique to each replicate and an additional random trinucleotide identifying individual DNA molecules was clipped but kept as metadata; 2. Reads with quality less than 20 for 10% or more of the bases were removed.3. We mapped the remaining reads to ...
iCLIP-seq: RNAs were extracted from immunoprecipitated protein-RNA complexes; for direct RNA sequencing, RNAs were extracted from cells using Trizol reagent (Invitrogen),iCLIP-seq libraries were prepared as described by Konig et al, NSMB (2010) 17: 909; the antibody used was purchased from Bethyl Laboratories (Cat#: A3...
GSM1003588
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186537,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01173954
GSM1003588
GSE40859
0.089459
HeLa
Public on Oct 01 2012
Sep 13 2012
9606
CstF64-iCLIP rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186537
https://www.ncbi.nlm.nih.gov/biosample/SAMN01173954
1
strategy: iCLIP-Seq,cell line: HeLa,clip antibody: CstF64
B228 Med Sci I
Irvine
USA
University of California, Irvine
Yongsheng,,Shi
iCLIP-seq: 1. Raw reads were demultiplexed using the sequencing barcode unique to each replicate and an additional random trinucleotide identifying individual DNA molecules was clipped but kept as metadata; 2. Reads with quality less than 20 for 10% or more of the bases were removed.3. We mapped the remaining reads to ...
iCLIP-seq: RNAs were extracted from immunoprecipitated protein-RNA complexes; for direct RNA sequencing, RNAs were extracted from cells using Trizol reagent (Invitrogen),iCLIP-seq libraries were prepared as described by Konig et al, NSMB (2010) 17: 909; the antibody used was purchased from Bethyl Laboratories (Cat#: A3...
GSM1003589
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX186538,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01173955
GSM1003589
GSE40859
0.073131
HeLa
Public on Oct 01 2012
Sep 13 2012
9606
CstF64-iCLIP rep3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX186538
https://www.ncbi.nlm.nih.gov/biosample/SAMN01173955
1
cell type: H1 embryonic stem cells
9 Cambridge Center
Cambridge
USA
Whitehead Institute for Biomedical Research
Richard,A,Young
For RNA-Seq samples. all sequenced reads were aligned to the human genome (Hg18) using the spliced read aligner TopHat version V1.4.0 (Trapnell et al. 2009). The paired end reads were aligned using "Bowtie -v" option for initial read mapping along with --microexon-search and --coverage-search options for identifying sp...
Total RNA was purified using mirVana miRNA isolation kit (Life Technologies) following the manufacturer instructions and treated with DNA-free™ DNase I (Ambion). Polyadenylated RNA was purified from the total RNA by two rounds of selection using Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA (...
GSM1006725
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE76586,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX188846,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01179603
GSM1006725
GSE41009
0.070537
RNA-seq H1-hESC
Public on Jan 02 2013
Sep 19 2012
9606
RNA_Seq_hESC_2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX188846
https://www.ncbi.nlm.nih.gov/biosample/SAMN01179603
1
cell type: H1 embryonic stem cells,drug treatment: Activin,drug concentration: 50ng/ml,duration of treatment: 48hr
9 Cambridge Center
Cambridge
USA
Whitehead Institute for Biomedical Research
Richard,A,Young
For RNA-Seq samples. all sequenced reads were aligned to the human genome (Hg18) using the spliced read aligner TopHat version V1.4.0 (Trapnell et al. 2009). The paired end reads were aligned using "Bowtie -v" option for initial read mapping along with --microexon-search and --coverage-search options for identifying sp...
Total RNA was purified using mirVana miRNA isolation kit (Life Technologies) following the manufacturer instructions and treated with DNA-free™ DNase I (Ambion). Polyadenylated RNA was purified from the total RNA by two rounds of selection using Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA (...
GSM1006726
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE76586,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX188847,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01179604
GSM1006726
GSE41009
0.136725
RNA-seq H1-hESC-48hr-Activin
Public on Jan 02 2013
Sep 19 2012
9606
RNA_Seq_48hr_1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX188847
https://www.ncbi.nlm.nih.gov/biosample/SAMN01179604
1
cell type: H1 embryonic stem cells,drug treatment: Activin,drug concentration: 50ng/ml,duration of treatment: 48hr
9 Cambridge Center
Cambridge
USA
Whitehead Institute for Biomedical Research
Richard,A,Young
For RNA-Seq samples. all sequenced reads were aligned to the human genome (Hg18) using the spliced read aligner TopHat version V1.4.0 (Trapnell et al. 2009). The paired end reads were aligned using "Bowtie -v" option for initial read mapping along with --microexon-search and --coverage-search options for identifying sp...
Total RNA was purified using mirVana miRNA isolation kit (Life Technologies) following the manufacturer instructions and treated with DNA-free™ DNase I (Ambion). Polyadenylated RNA was purified from the total RNA by two rounds of selection using Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA (...
GSM1006727
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE76586,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX188848,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01179605
GSM1006727
GSE41009
0.199196
RNA-seq H1-hESC-48hr-Activin
Public on Jan 02 2013
Sep 19 2012
9606
RNA_Seq_48hr_2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX188848
https://www.ncbi.nlm.nih.gov/biosample/SAMN01179605
1
cell type: Primary Human Umbilical Vein Endothelial Cells,passages: P3-6,treatment: VEGF,time: 0h
10 Shattuck St
Boston
USA
Harvard Medical School
Peter,J,Park
All ChIP-seq and Dnase-seqexperiments were aligned against hg19 using bowtie with the -m 1 option,All RNA-seq experiments were aligned against hg19 using tophat with the standard parameters and using Ensembl GRCH37 version 65 as a reference transcript database,FPKM for all RNA-seq experiments were performed using cuf...
Illumina multiplex library protocol modified to use 2 rather than 3 primers; DHS-seq protocol using modified, indexed primers.
GSM1009635
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX189728,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01731041
GSM1009635
GSE41166
0.002183
Primary Human Umbilical Vein Endothelial Cells
Public on Mar 31 2013
Sep 26 2012
9606
RNA-seq Hour 0
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX189728
https://www.ncbi.nlm.nih.gov/biosample/SAMN01731041
1
cell type: Primary Human Umbilical Vein Endothelial Cells,passages: P3-6,treatment: VEGF,time: 1h
10 Shattuck St
Boston
USA
Harvard Medical School
Peter,J,Park
All ChIP-seq and Dnase-seqexperiments were aligned against hg19 using bowtie with the -m 1 option,All RNA-seq experiments were aligned against hg19 using tophat with the standard parameters and using Ensembl GRCH37 version 65 as a reference transcript database,FPKM for all RNA-seq experiments were performed using cuf...
Illumina multiplex library protocol modified to use 2 rather than 3 primers; DHS-seq protocol using modified, indexed primers.
GSM1009636
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX189729,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01731042
GSM1009636
GSE41166
0.012346
Primary Human Umbilical Vein Endothelial Cells
Public on Mar 31 2013
Sep 26 2012
9606
RNA-seq Hour 1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX189729
https://www.ncbi.nlm.nih.gov/biosample/SAMN01731042
1
cell type: Primary Human Umbilical Vein Endothelial Cells,passages: P3-6,treatment: VEGF,time: 4h
10 Shattuck St
Boston
USA
Harvard Medical School
Peter,J,Park
All ChIP-seq and Dnase-seqexperiments were aligned against hg19 using bowtie with the -m 1 option,All RNA-seq experiments were aligned against hg19 using tophat with the standard parameters and using Ensembl GRCH37 version 65 as a reference transcript database,FPKM for all RNA-seq experiments were performed using cuf...
Illumina multiplex library protocol modified to use 2 rather than 3 primers; DHS-seq protocol using modified, indexed primers.
GSM1009637
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX189730,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01731043
GSM1009637
GSE41166
0.008229
Primary Human Umbilical Vein Endothelial Cells
Public on Mar 31 2013
Sep 26 2012
9606
RNA-seq Hour 4
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX189730
https://www.ncbi.nlm.nih.gov/biosample/SAMN01731043
1
cell type: Primary Human Umbilical Vein Endothelial Cells,passages: P3-6,treatment: VEGF,time: 12h
10 Shattuck St
Boston
USA
Harvard Medical School
Peter,J,Park
All ChIP-seq and Dnase-seqexperiments were aligned against hg19 using bowtie with the -m 1 option,All RNA-seq experiments were aligned against hg19 using tophat with the standard parameters and using Ensembl GRCH37 version 65 as a reference transcript database,FPKM for all RNA-seq experiments were performed using cuf...
Illumina multiplex library protocol modified to use 2 rather than 3 primers; DHS-seq protocol using modified, indexed primers.
GSM1009638
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX189731,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01731044
GSM1009638
GSE41166
0.011829
Primary Human Umbilical Vein Endothelial Cells
Public on Mar 31 2013
Sep 26 2012
9606
RNA-seq Hour 12
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX189731
https://www.ncbi.nlm.nih.gov/biosample/SAMN01731044
1
cell line: HL60,rna fraction: Total RNA
Craig L. Dobbin Genetics Research Centre,
St. John's
Canada
Memorial University of Newfoundland
Touati,,Benoukraf
Bases were called using Casava 1.8.4 with default settings.,76-bp paired-end reads were aligned to human genome (hg19) using tophat 2.0.2 with default settings and without a reference assembly.,Assemblies were generated using Cufflinks 2.0.2 again, without reference assembly,Overlap with DNMT1-RIP-Seq was calculated us...
Total RNA was extracted with TRI REAGENT® (MRC). Total RNA was extracted with TRI Reagent® (MRC). RNA samples were treated with DNase I (10 U of DNase I (Roche) per 3 ug of total RNA; 37ºC for one hour; in the presence of RNase inhibitor). Non-polyadenylated RNA fractions were selected with the MicroPoly (A) PuristT...
GSM1013480
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX190848,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01737708
GSM1013480
GSE32260,GSE41279
0.065361
Myeloid Leukemia
Public on Dec 01 2012
Oct 02 2012
9606
Total RNA
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX190848
https://www.ncbi.nlm.nih.gov/biosample/SAMN01737708
1
cell line: HL60,rna fraction: Non-polyadenylated RNA
Craig L. Dobbin Genetics Research Centre,
St. John's
Canada
Memorial University of Newfoundland
Touati,,Benoukraf
Bases were called using Casava 1.8.4 with default settings.,76-bp paired-end reads were aligned to human genome (hg19) using tophat 2.0.2 with default settings and without a reference assembly.,Assemblies were generated using Cufflinks 2.0.2 again, without reference assembly,Overlap with DNMT1-RIP-Seq was calculated us...
Total RNA was extracted with TRI REAGENT® (MRC). Total RNA was extracted with TRI Reagent® (MRC). RNA samples were treated with DNase I (10 U of DNase I (Roche) per 3 ug of total RNA; 37ºC for one hour; in the presence of RNase inhibitor). Non-polyadenylated RNA fractions were selected with the MicroPoly (A) PuristT...
GSM1013481
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX190849,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01737709
GSM1013481
GSE32260,GSE41279
0.034869
Myeloid Leukemia
Public on Dec 01 2012
Oct 02 2012
9606
Total RNA polyA(-)
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX190849
https://www.ncbi.nlm.nih.gov/biosample/SAMN01737709
1
cell line: HT29,cell type: colon cancer,treatment: control
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019735
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195293,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765636
GSM1019735
GSE41586,GSE41588
0
HT29 treated with 0 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 0 μM of 5-Aza, biological rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195293
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765636
1
cell line: HT29,cell type: colon cancer,treatment: control
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019736
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195294,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765637
GSM1019736
GSE41586,GSE41588
0
HT29 treated with 0 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 0 μM of 5-Aza, biological rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195294
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765637
1
cell line: HT29,cell type: colon cancer,treatment: control
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019737
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195295,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765638
GSM1019737
GSE41586,GSE41588
0
HT29 treated with 0 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 0 μM of 5-Aza, biological rep3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195295
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765638
1
cell line: HT29,cell type: colon cancer,treatment: 5-Aza low
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019738
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195296,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765639
GSM1019738
GSE41586,GSE41588
0.001323
HT29 treated with 5 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 5 μM of 5-Aza, biological rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195296
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765639
1
cell line: HT29,cell type: colon cancer,treatment: 5-Aza low
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019739
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195297,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765640
GSM1019739
GSE41586,GSE41588
0.00094
HT29 treated with 5 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 5 μM of 5-Aza, biological rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195297
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765640
1
cell line: HT29,cell type: colon cancer,treatment: 5-Aza low
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019740
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195298,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765641
GSM1019740
GSE41586,GSE41588
0.001323
HT29 treated with 5 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 5 μM of 5-Aza, biological rep3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195298
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765641
1
cell line: HT29,cell type: colon cancer,treatment: 5-Aza high
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019741
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195299,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765642
GSM1019741
GSE41586,GSE41588
0
HT29 treated with 10 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 10 μM of 5-Aza, biological rep1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195299
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765642
1
cell line: HT29,cell type: colon cancer,treatment: 5-Aza high
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019742
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195300,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765643
GSM1019742
GSE41586,GSE41588
0.000149
HT29 treated with 10 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 10 μM of 5-Aza, biological rep2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195300
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765643
1
cell line: HT29,cell type: colon cancer,treatment: 5-Aza high
Stony Brook University
Stony Brook
USA
Stony Brook University BME
Xiao,,Xu
Illumina Casava1.8 software used for basecalling.,Sequenced reads were trimmed for adaptor sequence and low quality bases (first 3 bases), then mapped to hg19 whole genome using tophatv 2.0.1 with parameters -r 10 -p 4,mapped reads from tophat were further quantifiled through Cufflink program (version 1.3.0),Fragments ...
Each monolayer was washed with PBS, scraped and centrifuged into a pellet. RNA was extracted from each pellet using Tri-Reagent according to the manufacturer's protocol.,Paired end 100 bp RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
GSM1019743
Illumina HiSeq 2000
Aug 08 2023
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195301,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01765644
GSM1019743
GSE41586,GSE41588
0.00431
HT29 treated with 10 μM of 5-Aza
Public on Sep 17 2013
Oct 15 2012
9606
HT29 at 10 μM of 5-Aza, biological rep3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195301
https://www.ncbi.nlm.nih.gov/biosample/SAMN01765644
1
cell type: corneal endothelial cells,age: 31y
Gonda BLDG Rm. 6554, P.O.Box 957088
Los Angeles
USA
UCLA
Kevin,,Huang
each sample was subjected to sequencing according to manufacturer’s instruction with Illumina Hiseq 2000. Reads were mapped to the hg19 genome using BWA.,For all datasets analyzed, we first transformed transcript read counts to the RPKM metric, and genes with an average RPKM<1 were filtered out, followed by quantile ...
RNA were extracted from those adult and fetal tissues using RNeasy Micro Kit (Qiagen, Cat. No. 74004, CA,USA). RNA concentration was detected by nanodrop spectrophotometer,RNA-Seq library construction followed the protocol described in illumina TruSeq™ RNA Sample Preparation Guide. Library construction was started w...
GSM1020212
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE81474,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195461,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01766527
GSM1020212
GSE41616
0.004722
corneal endothelial cells
Public on Jan 08 2013
Oct 16 2012
9606
adult CEC 33
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195461
https://www.ncbi.nlm.nih.gov/biosample/SAMN01766527
1
cell type: corneal endothelial cells,age: 56y
Gonda BLDG Rm. 6554, P.O.Box 957088
Los Angeles
USA
UCLA
Kevin,,Huang
each sample was subjected to sequencing according to manufacturer’s instruction with Illumina Hiseq 2000. Reads were mapped to the hg19 genome using BWA.,For all datasets analyzed, we first transformed transcript read counts to the RPKM metric, and genes with an average RPKM<1 were filtered out, followed by quantile ...
RNA were extracted from those adult and fetal tissues using RNeasy Micro Kit (Qiagen, Cat. No. 74004, CA,USA). RNA concentration was detected by nanodrop spectrophotometer,RNA-Seq library construction followed the protocol described in illumina TruSeq™ RNA Sample Preparation Guide. Library construction was started w...
GSM1020213
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE81474,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195462,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01766528
GSM1020213
GSE41616
0.007024
corneal endothelial cells
Public on Jan 08 2013
Oct 16 2012
9606
adult CEC 39
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195462
https://www.ncbi.nlm.nih.gov/biosample/SAMN01766528
1
cell type: corneal endothelial cells,age: 64y
Gonda BLDG Rm. 6554, P.O.Box 957088
Los Angeles
USA
UCLA
Kevin,,Huang
each sample was subjected to sequencing according to manufacturer’s instruction with Illumina Hiseq 2000. Reads were mapped to the hg19 genome using BWA.,For all datasets analyzed, we first transformed transcript read counts to the RPKM metric, and genes with an average RPKM<1 were filtered out, followed by quantile ...
RNA were extracted from those adult and fetal tissues using RNeasy Micro Kit (Qiagen, Cat. No. 74004, CA,USA). RNA concentration was detected by nanodrop spectrophotometer,RNA-Seq library construction followed the protocol described in illumina TruSeq™ RNA Sample Preparation Guide. Library construction was started w...
GSM1020214
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE81474,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195463,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01766529
GSM1020214
GSE41616
0
corneal endothelial cells
Public on Jan 08 2013
Oct 16 2012
9606
adult CEC 40
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195463
https://www.ncbi.nlm.nih.gov/biosample/SAMN01766529
1
cell type: corneal endothelial cells,age: gestation age 16-18wk
Gonda BLDG Rm. 6554, P.O.Box 957088
Los Angeles
USA
UCLA
Kevin,,Huang
each sample was subjected to sequencing according to manufacturer’s instruction with Illumina Hiseq 2000. Reads were mapped to the hg19 genome using BWA.,For all datasets analyzed, we first transformed transcript read counts to the RPKM metric, and genes with an average RPKM<1 were filtered out, followed by quantile ...
RNA were extracted from those adult and fetal tissues using RNeasy Micro Kit (Qiagen, Cat. No. 74004, CA,USA). RNA concentration was detected by nanodrop spectrophotometer,RNA-Seq library construction followed the protocol described in illumina TruSeq™ RNA Sample Preparation Guide. Library construction was started w...
GSM1020215
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE81474,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195464,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01766530
GSM1020215
GSE41616
0.006755
corneal endothelial cells
Public on Jan 08 2013
Oct 16 2012
9606
fetal CEC 1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195464
https://www.ncbi.nlm.nih.gov/biosample/SAMN01766530
1
cell type: corneal endothelial cells,age: gestation age 16-18wk
Gonda BLDG Rm. 6554, P.O.Box 957088
Los Angeles
USA
UCLA
Kevin,,Huang
each sample was subjected to sequencing according to manufacturer’s instruction with Illumina Hiseq 2000. Reads were mapped to the hg19 genome using BWA.,For all datasets analyzed, we first transformed transcript read counts to the RPKM metric, and genes with an average RPKM<1 were filtered out, followed by quantile ...
RNA were extracted from those adult and fetal tissues using RNeasy Micro Kit (Qiagen, Cat. No. 74004, CA,USA). RNA concentration was detected by nanodrop spectrophotometer,RNA-Seq library construction followed the protocol described in illumina TruSeq™ RNA Sample Preparation Guide. Library construction was started w...
GSM1020216
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
Reanalyzed by: GSE81474,SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX195465,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01766531
GSM1020216
GSE41616
0.074061
corneal endothelial cells
Public on Jan 08 2013
Oct 16 2012
9606
fetal CEC 2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX195465
https://www.ncbi.nlm.nih.gov/biosample/SAMN01766531
1
passage number: 5,cell type: fibroblast
230 South Frontage Road
New Haven
USA
Yale University
Flora,,Vaccarino
Base calling: Illumina CASAVA,Alignment: Tophat 1.3.1, parameters --solexa1.3-quals --coverage-search --no-novel-indels --microexon-search [extra parameter "-r 100" for PE 2x100 data, "-r 150" for 2x75 data and without "-r" parameter for SE data],Conversion from BAM to SAM: SAMtools 0.1.11,Conversion SAM to MRF: sam2mr...
Cells were treated with Accutase and pelleted. Total RNA was extracted using PicoPure kit (Life Technologies catalog # KIT0204).,Fibroblast RNA libraries for both families (03 and 1123) were prepared for sequencing using standard Illumina protocols using 5 ug of total RNA. iPSC RNA libraries for family 03 were prepare...
GSM1023063
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX198056,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01773270
GSM1023063
GSE41716
0.002273
fibroblast
Public on Oct 31 2012
Oct 19 2012
9606
RNA 03-02, F
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX198056
https://www.ncbi.nlm.nih.gov/biosample/SAMN01773270
1
passage number: 8,cell type: iPS
230 South Frontage Road
New Haven
USA
Yale University
Flora,,Vaccarino
Base calling: Illumina CASAVA,Alignment: Tophat 1.3.1, parameters --solexa1.3-quals --coverage-search --no-novel-indels --microexon-search [extra parameter "-r 100" for PE 2x100 data, "-r 150" for 2x75 data and without "-r" parameter for SE data],Conversion from BAM to SAM: SAMtools 0.1.11,Conversion SAM to MRF: sam2mr...
Cells were treated with Accutase and pelleted. Total RNA was extracted using PicoPure kit (Life Technologies catalog # KIT0204).,Fibroblast RNA libraries for both families (03 and 1123) were prepared for sequencing using standard Illumina protocols using 5 ug of total RNA. iPSC RNA libraries for family 03 were prepare...
GSM1023076
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX198069,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01773283
GSM1023076
GSE41716
0.019931
iPS
Public on Oct 31 2012
Oct 19 2012
9606
RNA S1123-01, i1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX198069
https://www.ncbi.nlm.nih.gov/biosample/SAMN01773283
1
passage number: 7,cell type: iPS
230 South Frontage Road
New Haven
USA
Yale University
Flora,,Vaccarino
Base calling: Illumina CASAVA,Alignment: Tophat 1.3.1, parameters --solexa1.3-quals --coverage-search --no-novel-indels --microexon-search [extra parameter "-r 100" for PE 2x100 data, "-r 150" for 2x75 data and without "-r" parameter for SE data],Conversion from BAM to SAM: SAMtools 0.1.11,Conversion SAM to MRF: sam2mr...
Cells were treated with Accutase and pelleted. Total RNA was extracted using PicoPure kit (Life Technologies catalog # KIT0204).,Fibroblast RNA libraries for both families (03 and 1123) were prepared for sequencing using standard Illumina protocols using 5 ug of total RNA. iPSC RNA libraries for family 03 were prepare...
GSM1023077
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX198070,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01773284
GSM1023077
GSE41716
0.011621
iPS
Public on Oct 31 2012
Oct 19 2012
9606
RNA S1123-01, i3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX198070
https://www.ncbi.nlm.nih.gov/biosample/SAMN01773284
1
passage number: 8,cell type: iPS
230 South Frontage Road
New Haven
USA
Yale University
Flora,,Vaccarino
Base calling: Illumina CASAVA,Alignment: Tophat 1.3.1, parameters --solexa1.3-quals --coverage-search --no-novel-indels --microexon-search [extra parameter "-r 100" for PE 2x100 data, "-r 150" for 2x75 data and without "-r" parameter for SE data],Conversion from BAM to SAM: SAMtools 0.1.11,Conversion SAM to MRF: sam2mr...
Cells were treated with Accutase and pelleted. Total RNA was extracted using PicoPure kit (Life Technologies catalog # KIT0204).,Fibroblast RNA libraries for both families (03 and 1123) were prepared for sequencing using standard Illumina protocols using 5 ug of total RNA. iPSC RNA libraries for family 03 were prepare...
GSM1023078
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX198071,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01773285
GSM1023078
GSE41716
0.016357
iPS
Public on Oct 31 2012
Oct 19 2012
9606
RNA S1123-01, i4
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX198071
https://www.ncbi.nlm.nih.gov/biosample/SAMN01773285
1
passage number: 6,cell type: iPS
230 South Frontage Road
New Haven
USA
Yale University
Flora,,Vaccarino
Base calling: Illumina CASAVA,Alignment: Tophat 1.3.1, parameters --solexa1.3-quals --coverage-search --no-novel-indels --microexon-search [extra parameter "-r 100" for PE 2x100 data, "-r 150" for 2x75 data and without "-r" parameter for SE data],Conversion from BAM to SAM: SAMtools 0.1.11,Conversion SAM to MRF: sam2mr...
Cells were treated with Accutase and pelleted. Total RNA was extracted using PicoPure kit (Life Technologies catalog # KIT0204).,Fibroblast RNA libraries for both families (03 and 1123) were prepared for sequencing using standard Illumina protocols using 5 ug of total RNA. iPSC RNA libraries for family 03 were prepare...
GSM1023080
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX198073,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01773287
GSM1023080
GSE41716
0.023139
iPS
Public on Oct 31 2012
Oct 19 2012
9606
RNA S1123-02, i11
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX198073
https://www.ncbi.nlm.nih.gov/biosample/SAMN01773287
1
passage number: 6,cell type: iPS
230 South Frontage Road
New Haven
USA
Yale University
Flora,,Vaccarino
Base calling: Illumina CASAVA,Alignment: Tophat 1.3.1, parameters --solexa1.3-quals --coverage-search --no-novel-indels --microexon-search [extra parameter "-r 100" for PE 2x100 data, "-r 150" for 2x75 data and without "-r" parameter for SE data],Conversion from BAM to SAM: SAMtools 0.1.11,Conversion SAM to MRF: sam2mr...
Cells were treated with Accutase and pelleted. Total RNA was extracted using PicoPure kit (Life Technologies catalog # KIT0204).,Fibroblast RNA libraries for both families (03 and 1123) were prepared for sequencing using standard Illumina protocols using 5 ug of total RNA. iPSC RNA libraries for family 03 were prepare...
GSM1023081
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX198074,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01773288
GSM1023081
GSE41716
0.032279
iPS
Public on Oct 31 2012
Oct 19 2012
9606
RNA S1123-02, i17
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX198074
https://www.ncbi.nlm.nih.gov/biosample/SAMN01773288
1
passage number: 9,cell type: iPS
230 South Frontage Road
New Haven
USA
Yale University
Flora,,Vaccarino
Base calling: Illumina CASAVA,Alignment: Tophat 1.3.1, parameters --solexa1.3-quals --coverage-search --no-novel-indels --microexon-search [extra parameter "-r 100" for PE 2x100 data, "-r 150" for 2x75 data and without "-r" parameter for SE data],Conversion from BAM to SAM: SAMtools 0.1.11,Conversion SAM to MRF: sam2mr...
Cells were treated with Accutase and pelleted. Total RNA was extracted using PicoPure kit (Life Technologies catalog # KIT0204).,Fibroblast RNA libraries for both families (03 and 1123) were prepared for sequencing using standard Illumina protocols using 5 ug of total RNA. iPSC RNA libraries for family 03 were prepare...
GSM1023082
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX198075,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01773289
GSM1023082
GSE41716
0.021789
iPS
Public on Oct 31 2012
Oct 19 2012
9606
RNA S1123-02, i2
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX198075
https://www.ncbi.nlm.nih.gov/biosample/SAMN01773289
1
strain: HUES 3Hb9::GFP,day of collection: Day 21 GFP high,treatment: SHH derived,cell type: FACS-purified motor neurons
52 Oxford St.
Cambridge
USA
Harvard University
Mackenzie,W.,Amoroso
The RNA samples sequenced on an Illumina HiSeq instrument at the HudsonAlpha Institute of Biotechnology.,The reads were aligned to the reference transcriptome as well as a library of exon junctions using Bowtie,Data was analyzed using Expression Plot using a P value of 0.0001 and a 2 fold change threshold,Gene ontology...
Trizol extraction of total RNA,NuGEN RNA kit for genomic sample amplification
GSM1024416
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX200689,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01779691
GSM1024416
GSE41795
0.070256
Human Stem Derived Motor Neurons GFP high
Public on Feb 06 2013
Oct 23 2012
9606
Positive Sample 1 SL16147
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX200689
https://www.ncbi.nlm.nih.gov/biosample/SAMN01779691
1
strain: HUES 3Hb9::GFP,day of collection: Day 21 GFP high,treatment: S+P derived,cell type: FACS-purified motor neurons
52 Oxford St.
Cambridge
USA
Harvard University
Mackenzie,W.,Amoroso
The RNA samples sequenced on an Illumina HiSeq instrument at the HudsonAlpha Institute of Biotechnology.,The reads were aligned to the reference transcriptome as well as a library of exon junctions using Bowtie,Data was analyzed using Expression Plot using a P value of 0.0001 and a 2 fold change threshold,Gene ontology...
Trizol extraction of total RNA,NuGEN RNA kit for genomic sample amplification
GSM1024417
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX200690,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01779692
GSM1024417
GSE41795
0.128037
Human Stem Derived Motor Neurons GFP high
Public on Feb 06 2013
Oct 23 2012
9606
Positive Sample 2 SL16145
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX200690
https://www.ncbi.nlm.nih.gov/biosample/SAMN01779692
1
strain: HUES 3Hb9::GFP,day of collection: Day 21 no GFP,treatment: S+P derived,cell type: FACS-purified motor neurons
52 Oxford St.
Cambridge
USA
Harvard University
Mackenzie,W.,Amoroso
The RNA samples sequenced on an Illumina HiSeq instrument at the HudsonAlpha Institute of Biotechnology.,The reads were aligned to the reference transcriptome as well as a library of exon junctions using Bowtie,Data was analyzed using Expression Plot using a P value of 0.0001 and a 2 fold change threshold,Gene ontology...
Trizol extraction of total RNA,NuGEN RNA kit for genomic sample amplification
GSM1024418
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX200691,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01779693
GSM1024418
GSE41795
0.021962
Human Stem Derived Motor Neurons no GFP
Public on Feb 06 2013
Oct 23 2012
9606
Negative Sample 1 SL16148
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX200691
https://www.ncbi.nlm.nih.gov/biosample/SAMN01779693
1
cell line: BJ cells,condition: Proliferation, normal conditions
Ramat Aviv
Tel Aviv
Israel
Tel Aviv University
Ran,,Elkon
Reads were aligned to a reference set of human transcripts using Bowtie,Number of reads mapping to each transcript was counted and normalized to FPKMs,Genome build: hg19,Supplementary_files_format_and_content: wig files represent read coverage at each genomic location (hg19), normalized to 10M reads
Total RNA was isolated using Trizol Reagent (Invitrogen), following the manufacturer's instructions. Poly(A) was isolated using the Oligotex mRNA mini kit (Qiagen). Libraries were prepared using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer's instructions.
GSM1041191
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX207917,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01821792
GSM1041191
GSE42509,GSE45833
0.003462
Immortalized primary fibroblasts
Public on Mar 24 2013
Nov 26 2012
9606
C1.rna
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX207917
https://www.ncbi.nlm.nih.gov/biosample/SAMN01821792
1
cell line: BJ cells,condition: Proliferation, normal conditions
Ramat Aviv
Tel Aviv
Israel
Tel Aviv University
Ran,,Elkon
Reads were aligned to a reference set of human transcripts using Bowtie,Number of reads mapping to each transcript was counted and normalized to FPKMs,Genome build: hg19,Supplementary_files_format_and_content: wig files represent read coverage at each genomic location (hg19), normalized to 10M reads
Total RNA was isolated using Trizol Reagent (Invitrogen), following the manufacturer's instructions. Poly(A) was isolated using the Oligotex mRNA mini kit (Qiagen). Libraries were prepared using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer's instructions.
GSM1041192
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX207918,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01821793
GSM1041192
GSE42509,GSE45833
0.004334
Immortalized primary fibroblasts
Public on Mar 24 2013
Nov 26 2012
9606
C2.rna
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX207918
https://www.ncbi.nlm.nih.gov/biosample/SAMN01821793
1
cell line: BJ cells,condition: Quiescence induced by serum depletion
Ramat Aviv
Tel Aviv
Israel
Tel Aviv University
Ran,,Elkon
Reads were aligned to a reference set of human transcripts using Bowtie,Number of reads mapping to each transcript was counted and normalized to FPKMs,Genome build: hg19,Supplementary_files_format_and_content: wig files represent read coverage at each genomic location (hg19), normalized to 10M reads
Total RNA was isolated using Trizol Reagent (Invitrogen), following the manufacturer's instructions. Poly(A) was isolated using the Oligotex mRNA mini kit (Qiagen). Libraries were prepared using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer's instructions.
GSM1041193
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX207919,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01821794
GSM1041193
GSE42509,GSE45833
0.008406
Immortalized primary fibroblasts
Public on Mar 24 2013
Nov 26 2012
9606
Q1.rna
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX207919
https://www.ncbi.nlm.nih.gov/biosample/SAMN01821794
1
cell line: BJ cells,condition: Quiescence induced by serum depletion
Ramat Aviv
Tel Aviv
Israel
Tel Aviv University
Ran,,Elkon
Reads were aligned to a reference set of human transcripts using Bowtie,Number of reads mapping to each transcript was counted and normalized to FPKMs,Genome build: hg19,Supplementary_files_format_and_content: wig files represent read coverage at each genomic location (hg19), normalized to 10M reads
Total RNA was isolated using Trizol Reagent (Invitrogen), following the manufacturer's instructions. Poly(A) was isolated using the Oligotex mRNA mini kit (Qiagen). Libraries were prepared using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer's instructions.
GSM1041194
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX207920,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01821795
GSM1041194
GSE42509,GSE45833
0.003264
Immortalized primary fibroblasts
Public on Mar 24 2013
Nov 26 2012
9606
Q2.rna
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX207920
https://www.ncbi.nlm.nih.gov/biosample/SAMN01821795
1
cell line: BJ cells,condition: pre-senescence; 5 dys after RASG12V induction,protocol: BJ cells expressing hTERT and tamoxifen-inducible RASG12V were cultured in the presence of 10-7M 4-OHT-Tamoxifen for 5 days
Ramat Aviv
Tel Aviv
Israel
Tel Aviv University
Ran,,Elkon
Reads were aligned to a reference set of human transcripts using Bowtie,Number of reads mapping to each transcript was counted and normalized to FPKMs,Genome build: hg19,Supplementary_files_format_and_content: wig files represent read coverage at each genomic location (hg19), normalized to 10M reads
Total RNA was isolated using Trizol Reagent (Invitrogen), following the manufacturer's instructions. Poly(A) was isolated using the Oligotex mRNA mini kit (Qiagen). Libraries were prepared using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer's instructions.
GSM1041195
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX207921,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01821796
GSM1041195
GSE42509,GSE45833
0.003264
Immortalized primary fibroblasts
Public on Mar 24 2013
Nov 26 2012
9606
preS1.rna
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX207921
https://www.ncbi.nlm.nih.gov/biosample/SAMN01821796
1
cell line: BJ cells,condition: pre-senescence; 5 dys after RASG12V induction,protocol: BJ cells expressing hTERT and tamoxifen-inducible RASG12V were cultured in the presence of 10-7M 4-OHT-Tamoxifen for 5 days
Ramat Aviv
Tel Aviv
Israel
Tel Aviv University
Ran,,Elkon
Reads were aligned to a reference set of human transcripts using Bowtie,Number of reads mapping to each transcript was counted and normalized to FPKMs,Genome build: hg19,Supplementary_files_format_and_content: wig files represent read coverage at each genomic location (hg19), normalized to 10M reads
Total RNA was isolated using Trizol Reagent (Invitrogen), following the manufacturer's instructions. Poly(A) was isolated using the Oligotex mRNA mini kit (Qiagen). Libraries were prepared using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer's instructions.
GSM1041196
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX207922,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01821797
GSM1041196
GSE42509,GSE45833
0.002747
Immortalized primary fibroblasts
Public on Mar 24 2013
Nov 26 2012
9606
preS2.rna
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX207922
https://www.ncbi.nlm.nih.gov/biosample/SAMN01821797
1
cell type: NTera2/D1,treatment: none
9500 Gilman Drive
La Jolla
USA
HHMI/UCSD
MICHAEL,G,ROSENFELD
The image analysis and base calling were performed by using Illumina's Genome Analysis pipeline.,The sequencing reads were aligned to hg18 using BFAST, and we kept only 1 aligned read/ genome position for downstream analyses.,An equal number of reads (4.4 million) was randomly extracted from each sample, and the sequen...
After harvesting the cells, RNA polymerases were allowed to run-on for 5 minutes at 30C (~100bp) in the presence of sarkosyl and BrUTP. The RNA was then base hydrolyzed and purified. BrUTP-labeled run-on RNA was immunopurified with anti-BrdUTP-coated agarose beads. Run-on RNA was then polyadenylated to generate primer ...
GSM1045177
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX206683,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01820058
GSM1045177
GSE42563,GSE42602
0.123891
NTera2/D1 cells
Public on Dec 29 2012
Nov 27 2012
9606
minusRA_GRO-seq
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX206683
https://www.ncbi.nlm.nih.gov/biosample/SAMN01820058
1
cell type: gastric adenocarcinoma cells,cell line: BGC-823
No.7 PengFei road
Shenzhen
China
Agricultural Genomes Institute at Shenzhen
Desheng,,Gong
Illumina BclConverter-1.9.0 software used for basecalling.,Adaptor sequences were removed, and low-quality sequence reads were trimmed. The clean reads of RNA sequencing reads were aligned to the reference genome (UCSC hg18) using the SOAP aligner with parameters -m 90 -x 500 -l 15 -s 35 -p 4,Reads Per Kilobase of exo...
Total RNA was isolated from BGC-823, AGS cells and YH cells using the miRNeasy Kit (Qiagen 74104) according to the manufacturer's protocol. An additional DNaseI digestion step was performed to ensure that the samples were not contaminated with genomic DNA. The RNA purity was assessed using the Agilent bioanalyzer. Tota...
GSM1056529
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX212298,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01840352
GSM1056529
GSE43093,GSE43096
0
gastric adenocarcinoma cells
Public on Jul 01 2014
Dec 21 2012
9606
BGC-823-RNA-seq
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX212298
https://www.ncbi.nlm.nih.gov/biosample/SAMN01840352
1
cell line: CNE1,cell type: human nasopharyngeal carcinoma cells,passages: 3,infected with: control lentivirus
Southern medical university
Guangzhou
China
Southern medical university
Xin,,Li
The original image data is transfered into sequence data by base calling, which is defined as raw data or raw reads and saved as fastq files.,To get the clean reads, removing the dirty raw reads is needed before data analysis. Filter steps: 1. Remove reads with adaptors; 2. Remove reads in whic...
Total RNA isolated with TRIzol reagent was treated with RNase-free DNase (NEB) at 37 oC for 10 minutes. The Dynabeads mRNA Purification Kit (Life Technologies) was used to isolate mRNA from the total RNA samples.,According to the manufacturer’s instructions, the mRNA was chemically fragmented by the use of divalent c...
GSM1057039
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX212471,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01853566
GSM1057039
GSE42945,GSE43126
0
CNE1-MOCK_RNA-seq
Public on Jun 30 2015
Dec 22 2012
9606
CNE1-MOCK
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX212471
https://www.ncbi.nlm.nih.gov/biosample/SAMN01853566
1
cell line: CNE1,cell type: human nasopharyngeal carcinoma cells,passages: 3,infected with: EBV-miRNA-BART1 lentivirus
Southern medical university
Guangzhou
China
Southern medical university
Xin,,Li
The original image data is transfered into sequence data by base calling, which is defined as raw data or raw reads and saved as fastq files.,To get the clean reads, removing the dirty raw reads is needed before data analysis. Filter steps: 1. Remove reads with adaptors; 2. Remove reads in whic...
Total RNA isolated with TRIzol reagent was treated with RNase-free DNase (NEB) at 37 oC for 10 minutes. The Dynabeads mRNA Purification Kit (Life Technologies) was used to isolate mRNA from the total RNA samples.,According to the manufacturer’s instructions, the mRNA was chemically fragmented by the use of divalent c...
GSM1057040
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX212472,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01853567
GSM1057040
GSE42945,GSE43126
0
CNE1-BART1_RNA-seq
Public on Jun 30 2015
Dec 22 2012
9606
CNE1-BART1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX212472
https://www.ncbi.nlm.nih.gov/biosample/SAMN01853567
1
cell line: CNE1,cell type: human nasopharyngeal carcinoma cells,passages: 3,infected with: EBV-miRNA-BART3 lentivirus
Southern medical university
Guangzhou
China
Southern medical university
Xin,,Li
The original image data is transfered into sequence data by base calling, which is defined as raw data or raw reads and saved as fastq files.,To get the clean reads, removing the dirty raw reads is needed before data analysis. Filter steps: 1. Remove reads with adaptors; 2. Remove reads in whic...
Total RNA isolated with TRIzol reagent was treated with RNase-free DNase (NEB) at 37 oC for 10 minutes. The Dynabeads mRNA Purification Kit (Life Technologies) was used to isolate mRNA from the total RNA samples.,According to the manufacturer’s instructions, the mRNA was chemically fragmented by the use of divalent c...
GSM1057041
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX212473,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01853568
GSM1057041
GSE42945,GSE43126
0
CNE1-BART3_RNA-seq
Public on Jun 30 2015
Dec 22 2012
9606
CNE1-BART3
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX212473
https://www.ncbi.nlm.nih.gov/biosample/SAMN01853568
1
cell line: CNE1,cell type: human nasopharyngeal carcinoma cells,passages: 3,infected with: EBV-miRNA-BART7 lentivirus
Southern medical university
Guangzhou
China
Southern medical university
Xin,,Li
The original image data is transfered into sequence data by base calling, which is defined as raw data or raw reads and saved as fastq files.,To get the clean reads, removing the dirty raw reads is needed before data analysis. Filter steps: 1. Remove reads with adaptors; 2. Remove reads in whic...
Total RNA isolated with TRIzol reagent was treated with RNase-free DNase (NEB) at 37 oC for 10 minutes. The Dynabeads mRNA Purification Kit (Life Technologies) was used to isolate mRNA from the total RNA samples.,According to the manufacturer’s instructions, the mRNA was chemically fragmented by the use of divalent c...
GSM1057042
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX212474,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01853569
GSM1057042
GSE42945,GSE43126
0
CNE1-BART7_RNA-seq
Public on Jun 30 2015
Dec 22 2012
9606
CNE1-BART7
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX212474
https://www.ncbi.nlm.nih.gov/biosample/SAMN01853569
1
cell type: iPSC
1300 Morris Park Ave., F103
Bronx
USA
Albert Einstein College of Medicine
Herb,,Lachman
Illumina Casava1.7 software used for basecalling.,RNA-Seq reads were aligned to the human genome (GRCh37/hg19) using the software TopHat (version 1.3.2 ),The resulting alignment data from Tophat were then fed to an assembler, Cufflinks (version 1.3.0) to assemble aligned RNA-Seq reads into transcripts,the program Cuff...
Total RNA was isolated from cells using the miRNeasy Kit (Qiagen) according to the manufacturer’s protocol. An additional DNAse1 digestion step was performed to ensure that the samples were not contaminated with genomic DNA. RNA purity was assessed using the Agilant 2100 Bioanalyzer (Beijing Genomics Institute). Eac...
GSM1057333
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX212595,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01860731
GSM1057333
GSE43143
0.016421
iPSC
Public on Jan 01 2014
Dec 26 2012
9606
tips_rna_seq
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX212595
https://www.ncbi.nlm.nih.gov/biosample/SAMN01860731
1
cell type: NPC
1300 Morris Park Ave., F103
Bronx
USA
Albert Einstein College of Medicine
Herb,,Lachman
Illumina Casava1.7 software used for basecalling.,RNA-Seq reads were aligned to the human genome (GRCh37/hg19) using the software TopHat (version 1.3.2 ),The resulting alignment data from Tophat were then fed to an assembler, Cufflinks (version 1.3.0) to assemble aligned RNA-Seq reads into transcripts,the program Cuff...
Total RNA was isolated from cells using the miRNeasy Kit (Qiagen) according to the manufacturer’s protocol. An additional DNAse1 digestion step was performed to ensure that the samples were not contaminated with genomic DNA. RNA purity was assessed using the Agilant 2100 Bioanalyzer (Beijing Genomics Institute). Eac...
GSM1057334
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
total RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX212596,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01860732
GSM1057334
GSE43143
0.009923
NPC
Public on Jan 01 2014
Dec 26 2012
9606
NPC_rna_seq
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX212596
https://www.ncbi.nlm.nih.gov/biosample/SAMN01860732
1
cell line: MDA-MB-231,treatment: CXCL12 (0ng/mL) + IGF1 (0ng/mL)
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, cells were harvested in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated with RNAqeuous micro kit (Life Technologies).,RNA sequencing l...
GSM1060226
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215394,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883067
GSM1060226
GSE43296
0.003616
cells in culture
Public on Nov 01 2013
Jan 04 2013
9606
0+0_a
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215394
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883067
1
cell line: MDA-MB-231,treatment: CXCL12 (0ng/mL) + IGF1 (0ng/mL)
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, cells were harvested in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated with RNAqeuous micro kit (Life Technologies).,RNA sequencing l...
GSM1060227
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215395,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883068
GSM1060227
GSE43296
0.004891
cells in culture
Public on Nov 01 2013
Jan 04 2013
9606
0+0_b
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215395
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883068
1
cell line: MDA-MB-231,treatment: CXCL12 (30ng/mL) + IGF1 (10ng/mL)
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, cells were harvested in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated with RNAqeuous micro kit (Life Technologies).,RNA sequencing l...
GSM1060228
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215396,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883069
GSM1060228
GSE43296
0.022274
cells in culture
Public on Nov 01 2013
Jan 04 2013
9606
30+10_a
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215396
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883069
1
cell line: MDA-MB-231,treatment: CXCL12 (30ng/mL) + IGF1 (10ng/mL)
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, cells were harvested in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated with RNAqeuous micro kit (Life Technologies).,RNA sequencing l...
GSM1060229
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215397,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883070
GSM1060229
GSE43296
0.008008
cells in culture
Public on Nov 01 2013
Jan 04 2013
9606
30+10_b
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215397
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883070
1
cell line: MDA-MB-231,treatment: CXCL12 (300ng/mL) + IGF1 (100ng/mL)
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, cells were harvested in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated with RNAqeuous micro kit (Life Technologies).,RNA sequencing l...
GSM1060230
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215398,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883071
GSM1060230
GSE43296
0.021771
cells in culture
Public on Nov 01 2013
Jan 04 2013
9606
300+100_a
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215398
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883071
1
cell line: MDA-MB-231,treatment: CXCL12 (300ng/mL) + IGF1 (100ng/mL)
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, cells were harvested in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated with RNAqeuous micro kit (Life Technologies).,RNA sequencing l...
GSM1060231
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215399,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883072
GSM1060231
GSE43296
0.023018
cells in culture
Public on Nov 01 2013
Jan 04 2013
9606
300+100_b
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215399
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883072
1
cell line: EGFP-10a+ MDA-MB-231,protocol: supplemented with 1:1 ratio of MSC
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, the whole tumor were harvested and cut into small pieces. Tumor slices were lysed in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated w...
GSM1060352
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215400,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883073
GSM1060352
GSE43306
0.076879
mammary tumor xenograft
Public on Nov 01 2013
Jan 06 2013
9606
M1
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215400
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883073
1
cell line: EGFP-10a+ MDA-MB-231,protocol: supplemented with 1:1 ratio of MSC
417 East 68th Street
New York
USA
Memorial Sloan-Kettering Cancer Center
Xin,,Jin
Base-calling was performed with Illumina Casava v1.8.1,Sequenced reads were mapped to hg19 of refseq from UCSC with TopHat v1.4.0,Gene-level expression counts were determined with HTSeq,Genome_build: hg19,Supplementary_files_format_and_content: tab-delimited text files that include gene-level expression counts
Polysome-associated RNA was extracted following the TRAP protocol from Heiman et al, Cell, 2009. Briefly, the whole tumor were harvested and cut into small pieces. Tumor slices were lysed in polysome extraction buffer. Polysomes were pulled down by anti-GFP coated sepharose beads. Polysome-associated RNA was isolated w...
GSM1060353
Illumina HiSeq 2000
May 15 2019
cDNA
transcriptomic
RNA-Seq
polyA RNA
Homo sapiens
GPL11154
SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX215401,BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN01883074
GSM1060353
GSE43306
0.005638
mammary tumor xenograft
Public on Nov 01 2013
Jan 06 2013
9606
M6
SRA
https://www.ncbi.nlm.nih.gov/sra?term=SRX215401
https://www.ncbi.nlm.nih.gov/biosample/SAMN01883074
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